"

Cookies ussage consent

I agree.

Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our site without changing the browser settings you grant us permission to store that information on your device.

The effect of photoresist thin film on cyclic voltammetry measurement for DNA immobilization and hybridization of indicator-free DNA sensor

I. H. HAMZAH1,2,3,* , C. Y. YU4, ASRULNIZAM ABD MANAF1,2, O. SIDEK1,2, Y. Y. CHAN4

Affiliation

  1. Collaborative microElectronic Design Excellence Centre (CEDEC), Universiti Sains Malaysia, Engineering Campus, 14300, Penang, Malaysia
  2. School of Electrical and Electronic Engineering, Universiti Sains Malaysia, Engineering Campus, 14300, Penang, Malaysia
  3. Faculty of Electrical Engineering, UniversitiTeknologi MARA, 40450, Malaysia
  4. School of Medical Sciences, UniversitiSains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia

Abstract

The effect of positive photoresist thin film fabricated between electrodes on the glass slide is the focus in the present study. A 1.04 mm2 electrode size is used and the cyclic voltammetry (CV) of 0.02M K3Fe(CN)6 in 0.1M KCl has been analyzed throughout the work for Au bare electrodes, DNA probe immobilization and DNA target hybridization. An adhesive bonding method of polydimethylsiloxane (PDMS) is implemented. The atomic force microscope (AFM) analysis on the root mean square (RMS) value is then carried out to prove that the surface roughness and electrode thickness do not influence the analysis.

Keywords

Photoresist thin film, Biomaterial, Biosensor, Electrochemical, PDMS.

Citation

I. H. HAMZAH, C. Y. YU, ASRULNIZAM ABD MANAF, O. SIDEK, Y. Y. CHAN, The effect of photoresist thin film on cyclic voltammetry measurement for DNA immobilization and hybridization of indicator-free DNA sensor, Optoelectronics and Advanced Materials - Rapid Communications, 4, 12, December 2010, pp.2103-2107 (2010).

Submitted at: Nov. 18, 2010

Accepted at: Nov. 29, 2010