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Synthesis and characterization of TiO2 nanoparticles and investigation of their in vitro action against Staphylococcus aureus (PTCC 1189)

ALI ABDOLAHZADEH ZIABARI1,* , SAJIHE BAHREKAZEMI2, MOHAMMAD FAEZI GHASEMI2, KHOSRO ISSAZADEH2

Affiliation

  1. Nano Research Lab, Lahijan Branch, Islamic Azad University, P.O. Box1616, Lahijan, Iran
  2. Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran

Abstract

Titanium dioxide (TiO2) nanopowders in different concentrations were successfully synthesized via sol-gel route. The products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). The samples were polycrystalline in nature with anatase, rutile or a mixture of anatase and rutile phases. The diameter of nanoparticles was estimated as being: 28.70, 31.05, and 32.94 nm for 0.1, 0.3, and 1.0 molar concentration, respectively. The sharp peaks in FTIR spectrum determined the purity of TiO2 nanopowders. Antibacterial action of the products against Staphylococcus aureus (PTCC 1189) was evaluated by colony counting method. The photo catalytic property of TiO2 nanopowders was investigated following inactivation of Staphylococcus aureus (PTCC 1189) by irradiation with a UV-B lamp. The TiO2 nanopowders have a main antibacterial effect on S. aureus (PTCC 1189) at concentration of 1 mg/mL. Increasing the molarity from 0.1 to 1.0 M has decreased the viable cell concentration of S. aureus (PTCC 1189) from 95.21 % to 6.73 % after 3 hours..

Keywords

Staphylococcus aureus (PTCC 1189), TiO2 nanopowder, Sol-gel, Antibacterial activity.

Citation

ALI ABDOLAHZADEH ZIABARI, SAJIHE BAHREKAZEMI, MOHAMMAD FAEZI GHASEMI, KHOSRO ISSAZADEH, Synthesis and characterization of TiO2 nanoparticles and investigation of their in vitro action against Staphylococcus aureus (PTCC 1189), Optoelectronics and Advanced Materials - Rapid Communications, 10, 5-6, May-June 2016, pp.422-425 (2016).

Submitted at: March 11, 2015

Accepted at: June 9, 2016