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Silver-chitosan nanobiocomposite as urea biosensor

AKBAR ALI1, MUHAMMAD FAKHAR-E-ALAM2, NAJEEB ABBAS2, ZAFAR WAZIR1, MAGNUS WILLANDER3, MUHAMMAD TUFAIL1, M. ATIF4,5,*

Affiliation

  1. Faculty of Basic Sciences, Riphah International University, Islamabad
  2. Department of Physics, Faculty of Science and Technology, GC University, Faisalabad, Pakistan
  3. Department of Science and Technology, Campus Norrköping, Linköping University, SE-601 74 Norrköping, Sweden
  4. Department of Physics and Astronomy, King Saud University, Riyadh, Saudi Arabia
  5. National Institute of Laser and Optronics, Nilore, Islamabad, Pakistan

Abstract

Silver nanoparticles (Ag NPs) were synthesized by aqueous chemical growth technique. The above mentioned synthesized materials were characterized by applying scanning electron microscope (SEM) and X-ray diffraction for confirmation of morphological analysis, compositional purity, and crystalline property and emission characteristics as well. In order to fabricate the urea biosensor (potentiometric), a solution of deionized water and chitosan was prepared having Ag NPs. The said solution was dropped on the glass fiber filter having diameter of 2 cm. A wire of copper having thickness of approximately 500 μm was used for the voltage signal to pull out from the said working nanoparticles (NPs). To improve the strength, sensitivity and the quality of the potentiometric urea biosensor, a specific functional surface of Ag NPs was attained by electrostatic restrained of an enzyme (urease) onto the chitosan-Ag (a nanobiocomposite). The potentiometric reaction was measured via electrochemical detection technique. The potentiometric urea biosensor illustrates significant sensibility at room temperature with ≈ 42 mV as per span. Furthermore, the said biosensor showed an appropriate stable response within 7 sec..

Keywords

Ag nanoparticles (Ag NPs), Nanobiocomposite, Urea biosensor, Scanning electron microscope (SEM), X-ray diffraction.

Citation

AKBAR ALI, MUHAMMAD FAKHAR-E-ALAM, NAJEEB ABBAS, ZAFAR WAZIR, MAGNUS WILLANDER, MUHAMMAD TUFAIL, M. ATIF, Silver-chitosan nanobiocomposite as urea biosensor, Optoelectronics and Advanced Materials - Rapid Communications, 8, 11-12, November-December 2014, pp.1238-1242 (2014).

Submitted at: Sept. 27, 2014

Accepted at: Nov. 13, 2014