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Passive nonspecific labeling of cyanobacteria in natural samples using quantum dots

A. ARMĂŞELU1, A. POPESCU2, I. APOSTOL3, I. ARDELEAN4, V. DAMIAN3, I. IORDACHE3, I. SARCHIZIAN4, D. APOSTOL3,*

Affiliation

  1. Transilvania University of Brasov, Department of Physics, Eroilor str. 29, Brasov, Romania
  2. Faculty of Physics, University of Bucharest, Atomistilor 405, Magurele, Ilfov, Romania
  3. National Institute for Lasers Plasma and Radiation Physics, Atomistilor 409, PO Box MG-36, Măgurele 077125, Ilfov, Romania
  4. Institute of Biology, Centre of Microbiology, Splaiul Independentei 296, Bucharest 060031, Romania

Abstract

The aim of this paper is to investigate the nonspecific labeling of cyanobacteria in biological samples with quantum dots (QDs). We focused on some interesting features of interaction between CdSe/ZnS QDs and filamentous and unicellular cyanobacteria from marine and sulfurous mesothermal springs. The fluorescence microscope images indicated that the labeling of the unicellular cyanobacteria with QDs did not take place, but in exchange, these QDs migrated in the direction of filamentous cyanobacteria and remained fixed on their sheaths. Based on digital epifluorescence image analysis, we tried to explain the color changes of cyanobacterial filament labeled with QDs, by adding supplementary QD quantities to the cyanobacteria cultures..

Keywords

Quantum dots, Cyanobacteria, Nonspecific labeling, Epifluorescence color changes, Digital analysis.

Citation

A. ARMĂŞELU, A. POPESCU, I. APOSTOL, I. ARDELEAN, V. DAMIAN, I. IORDACHE, I. SARCHIZIAN, D. APOSTOL, Passive nonspecific labeling of cyanobacteria in natural samples using quantum dots, Optoelectronics and Advanced Materials - Rapid Communications, 5, 10, October 2011, pp.1084-1090 (2011).

Submitted at: Sept. 9, 2011

Accepted at: Oct. 20, 2011