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Isocratic reverse phase HPLC estimation method of Torsemide and Spironolactone in pharmaceutical combined dosage form

SMITA SHARMA1,* , M. C. SHARMA2, D. V. KOHLI3, S. C. CHATURVEDI4

Affiliation

  1. Department of Chemistry Yadhunath Mahavidyalya Bhind (M.P) 477001 India
  2. School of Pharmacy, Devi Ahilya Vishwavidyalaya, Khandwa Road, Indore (M.P)-452001, India
  3. Department of Pharmaceutical Sciences Dr.H.S.G.University Sagar (M.P) 470003 India
  4. Shri Arvindo Institute of Pharmacy Ujjain Road Indore (M.P) 453111 India

Abstract

Validated high performance liquid chromatographic (HPLC) method for estimation of Torsemide (TOR) and Spironolactone (SPI) in tablet dosage form. Isocratic RP-HPLC separation was achieved on Licrosphere C18 column (250 x 4.6mm) using Methanol: Acetonitrile: Phosphate buffer, pH 6.5 (60:20:20 v/v) at flow rate of 1.5 ml/min at 30 oC temperature. Quantitation was achieved by UV detection at 252 nm over the conc. range 0-25 μg/ml for both the drugs with mean recoveries of 100.01% + 0.12 and 100.64% + 0.20 for TOR and SPI respectively. This method is simple, precise and sensitive and applicable for the simultaneous estimation of TOR and SPI in tablet pharmaceutical combined dosage form..

Keywords

Pharmaceutical, Torsemide, Spironolactone, HPLC.

Citation

SMITA SHARMA, M. C. SHARMA, D. V. KOHLI, S. C. CHATURVEDI, Isocratic reverse phase HPLC estimation method of Torsemide and Spironolactone in pharmaceutical combined dosage form, Optoelectronics and Advanced Materials - Rapid Communications, 4, 2, February 2010, pp.234-237 (2010).

Submitted at: Nov. 24, 2009

Accepted at: Feb. 2, 2010