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M. E. BARBINTA-PATRASCU1, A. M. IORDACHE2,3,*
This paper reports the investigation of the interaction between the food additive amaranth (azorubine S, E123) and methylene blue-marked-DNA, monitored by UV-Vis absorption and fluorescence emission spectroscopy, as well as cyclic voltammetry. The binding constants (Kb) for the amaranth with marked-DNA was estimated to be 2×107 M-1 through absorption spectroscopy, and 1.1×107 M−1 through fluorescence analysis. The cyclic voltammetry method showed that both anodic and cathodic current peaks of amaranth decreased upon addition of the DNA. These findings could be exploited to design innovative DNA-based materials for biosensing and biophotonic applications..
Amaranth dye (azorubine S, E123), DNA, Methylene blue, Spectral and electrochemical characterization.
M. E. BARBINTA-PATRASCU, A. M. IORDACHE, Biophysical aspects on interaction between DNA and the food dye - amaranth (azorubine S, E123), Optoelectronics and Advanced Materials - Rapid Communications, 13, 11-12, November-December 2019, pp.624-628 (2019).
Submitted at: Nov. 25, 2019
Accepted at: Dec. 10, 2019
Optoelectronics and Advanced Materials - Rapid Communications
(2022): 0.5 - 2022 Journal Citation Reports (Clarivate Analytics, 2023)
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