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I. GRUIA1,* , S. B. YERMOLENKO2, M. I. GRUIA3, P. V. IVASHKO2, T. ŞTEFĂNESCU4
Early detection of malignant transformation is a goal of modern medicine and to this end there is an impressive number of approaches from the scientific field that tries to identify early changes preceding malignant transformation in order to establish a correct diagnosis. This paper aims is to combine the optical and biochemical techniques for identifying the changes in membrane dynamics of growth and development of experimental solid tumours. During the purpose has been used experimental Walker 256 carcinoma graft in Wistar rats, followed from day 7 up to day 24 from inoculation of tumour cells. Optical techniques were used fractalometry laser polarization in the preclinical diagnosis of pathological changes and degenerative-dystrophy of experimental tissue, and in terms of biochemical tumour tissue was determined the reaction of lipid peroxidation monitored by the malondialdehyde (MDA), the end product assays of reaction. In addition were followed also the total antioxidants as a response of the endogenous defences systems. The results indicate a rising profile of the processes investigated by the 14th day after tumour graft, following a decrease due to lack of substrate enzymatic reactions, specifically the double links of polyunsaturated fatty acids in the membranes change during tumour development.These data are consistent with the changes of the optical investigated parameters.In conclusion, it can be said that a combination of optical and biochemical methods can provide additional data in order to determine early cellular changes occurring in malignant transformed cells. To track these changes in experimental tumour growth dynamics is useful for determining the optimal timing of oncostatic treatment intervention..
Fractalometry, Membranes modifications, Dichroism, Maligned tissue.
I. GRUIA, S. B. YERMOLENKO, M. I. GRUIA, P. V. IVASHKO, T. ŞTEFĂNESCU, Spectral and biochemical methods for identification of cellular and tissues malignant changes, Optoelectronics and Advanced Materials - Rapid Communications, 4, 4, April 2010, pp.523-526 (2010).
Submitted at: Feb. 5, 2010
Accepted at: April 11, 2010
Optoelectronics and Advanced Materials - Rapid Communications
(2025): Impact Factor = 0.5 - Journal Citation Reports (Clarivate Analytics, 2026)
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